PiggyBac inducible gene expression vector system combines Creative BioMart's efficient piggyBac vector system and Tet-on inducible gene expression system to help you realize the permanent integration of Tetracycline-inducible gene expression cassette based on transfection with the host genome.
Our tet-on inducible gene expression vector aims to achieve almost complete silence of GOI in the absence of tetracycline and its analogues (e.g., doxycycline) and respond to the addition of tetracycline or one of its analogues (e.g., doxycycline).
Our piggyBac inducible gene expression system contains two vectors, both of which are designed as E. coli plasmids.
A tetracycline-inducible gene expression cassette consisting of a tetracycline response element (TRE) promoter driving the user-selected GOI and a ubiquitous or tissue-specific promoter driving the regulatory protein TTS/rtTA is cloned into this region.
When the helper plasmid and transposon plasmid are cotransfected into the target cells, the transposase produced by the helper cells will recognize the two TRS on the transposon and insert the flanking region containing the two TRS into the host genome. Insertion usually occurs at the host chromatid site containing the ttaa sequence, which repeats on both sides of the integrated fragment. Then gene expression can be turned on in the presence of tetracycline.
Switch-like gene activation: Our Tet-On gene expression vectors act as a true tetracycline-regulated on-and-off switch for controlling gene expression, which can minimize the background expression without induction and result in high sensitivity and high dynamic range of the tetracycline induction.
Permanent integration of vector DNA: Conventional transfection results in almost entirely transient delivery of DNA into host cells due to the loss of DNA over time.
Technical simplicity: The piggyBac inducible gene expression vector can be introduced into mammalian cells by conventional transfection. Delivering plasmid vectors into cells by conventional transfection is technically straightforward, and far easier than virus-based vectors which require the packaging of live viruses.
Very large cargo space: Our transposon vector can accommodate ~30 kb of total DNA. The plasmid backbone, transposon-related sequences and the Tet-On components occupy only about 4.6 kb, leaving plenty of room to accommodate the user's sequence of interest.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
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