Autodisplay's enzyme spectrum on cell surface includes hydrolase, oxidoreductase and electron transfer protein. Creative BioMart is now combining autodisplay technology with immobilization technology to develop new biocatalysts for our customers. Compared with the purified enzyme, our service is expected to save customers up to 80% of the primary cost.
E. coli cells with specific enzymes on their surface can be used as biocatalysts for the production of fine chemicals. Benefitting from the mobility of AIDA-I anchoring domain in the outer membrane of E. coli, both monomeric enzymes and polymerases can be used for surface display.
In addition, different enzymes can also be expressed on the same cell surface by using autodisplay technology. These enzymes can interact with each other to improve the overall catalytic activity of cells.
This technology can also be used to screen the specific characteristics such as catalytic activity or stability of a specific enzyme. Different variants of this protein/enzyme are autodisplayed on the surface of each E. coli cell, and a diverse library is established for subsequent directional screening.
At present, Creative BioMart has used autodisplay technology to display a variety of enzymes, including hydrolase, esterase, sorbitol dehydrogenase, and bovine adrenodoxin.
β - lactamase has been successfully expressed. A large number of β - lactamases were detected on the cell surface, and the cell integrity remained intact.
Esterases, which exhibit a wide range of natural diversity in substrates and reaction types, have been widely used in biotechnology and industrial applications. EstA has been successfully demonstrated. After the display, the activity of E. coli did not decrease, which indicated that the advantage of hospital surface display of esterase by autodisplay technology was obvious.
It is difficult to produce polyols and sugars through standard organic synthesis, because there are a lot of the same functional groups in different positions of these molecules. Therefore, the use of enzymes in biocatalysis has high regioselectivity and stereoselectivity, and the solution can be obtained. For this purpose, SDH from Rhodococcus sphaericus was expressed on the cell surface by autodisplay.
The ferredoxin from bovine adrenal cortex, called adrenaline (ADX, 14.4 kDa), belongs to [2Fe – 2S] ferredoxin, which is a small acidic ferrithionein family.
As a single fusion protein, it is expressed on the surface of E. coli and forms dimer ADX molecule efficiently and spontaneously on the surface of bacteria. A whole-cell biocatalyst system was developed by adding purified adrenotoxin reductase and P450 CYP11A1, which effectively synthesized pregnenolone from cholesterol
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Reference
Jose, J. Autodisplay: efficient bacterial surface display of recombinant proteins. Appl Microbiol Biotechnol 69, 607-614 (2006).
