Lentivirus Mediated Tet-inducible System

Creative BioMart's lentiviral tet inducible gene expression vector combines the efficient third-generation lentiviral vector system with a tet inducible gene expression system to help you permanently integrate the tetracycline-inducible gene expression cassette into the host genome.

Technology Description

Lentiviral vector system is an efficient vector, which can introduce genes into mammalian cells permanently. Our specific expression ideas are as follows

• When the virus is added to the target cell, the RNA cargo is shuttled into the cell, where it is reverse transcribed into DNA and randomly integrated into the host genome. The inducible expression cassette placed between the two LTRs during vector construction is inserted into the host DNA permanently together with the rest of the virus genome.

Although our lentiviral tet inducible gene expression vector includes an inducible gene expression cassette composed of the tre promoter driving the user-selected GOI, a separate helper vector must be used to provide tre binding regulatory proteins TTS and rtTA for tetracycline-induced gene expression. In the absence of tetracycline, the leakage expression is minimized. For the lentiviral inducible gene expression vector system, compared with the multi-in-one vector system, the double vector system can achieve a higher level of transgenic induction in the presence of tetracycline.

Why Choose This System

When coexpressed with the Tet regulatory proteins tTS and rtTA, our lentivirus Tet inducible gene expression vector can achieve nearly complete silencing of the GOI in the absence of tetracycline, and strong, rapid expression in response to the addition of tetracycline. The lentiviral Tet inducible gene expression vector is derived from the third-generation lentiviral vector system. It is optimized for high copy number replication in E. coli, high-titer packaging of live virus, efficient viral transduction of a wide range of cells, efficient vector integration into the host genome, and high-level transgene expression.

• Switch-like gene activation
• High-level expression
• Permanent integration of vector DNA
• High viral titer
• Very broad tropism
• Relative uniformity of gene delivery
• Effectiveness in vitro and in vivo
• Safety

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