Insect virus (baculovirus) is an efficient media to deliver foreign genes to the host mammalian cells. This method is named as BacMam. One of the speed limit steps is the construction of an expression vector. To improve the expression efficiency, Creative BioMart now offers Gateway recombination-based BacMam expression service, through which the insect cell-infectious vector can be formed within an hour. Our technology overview and service content are listed below. For any inquiry or application, please submit an online inquiry or contact us.
Gateway is based on the integration of phage DNA into the host bacterial genome. This technology can facilitate the insertion of the gene of interest into the baculovirus genome in vitro through the LR recombination, eliminating the section of transforming bacteria, bacmid extraction, or co-transfection. Through the Gateway recombination reaction, the recombinant baculovirus genome needed for transfection can be obtained within one hour, dramatically shortening the protein expression period.
The particularity of this system is mainly shown in its vector—— BacMam pCMV-DEST Vector. The technology adopted both Tn7 transposition and LR recombination for rapid subclone construction.
In order to be identified by mammalian cells, an hCMV-IE1 promoter (or other mammalian promoters) is previously inserted into the donor vectors and it will be integrated into the baculovirus together with other elements as well as the gene of interest.
| Service Name | Service Description | Timeline | Deliverables |
| Gene Synthesis |
|
> 1 week |
|
| Prepare Entry Clone |
|
5 mins-day |
|
| Prepare Recombinant Bacmid |
|
> 1 week |
|
| Recombinant Baculovirus Production |
|
1 week |
|
| Mammalian cell transduction and protein expression |
|
1.5 weeks |
|
If you have any questions or requests, please feel free to contact us or submit an online inquiry. Our customer service is always ready for you 24/7.
